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Control of Human Aging With Special Nutritional Program.
Presentation of the Method
By Arcady L. Economo. Ph.D, Pharm.D. Anti-Aging Center Europe

_______________________________________________________________

Key words: aging, dietary restriction, nutritional program, human life span extension
_______________________________________________________________

ABSTRACT
Dietary restriction proved to be extremely effective for extending maximum life span in animals. Are the animal data translatable to humans? Modern scientific data indicates that with high order of probability we can answer on this question yes ! If yes than we ought to change our diet. The purpose of this work was to develop special nutrient criteria for human being - Anti-Aging Dietary Norms (AADN) for construction of various anti-aging food regimes - that are low-calorie, properly balanced and individually adjusted. 42 Versions of AADN were developed on the basis of present scientific data on biology of aging process: 7 versions for Standard model, 9 versions for Diabetic model, 17 versions for Gastroentheropathy model and 9 versions for Ischemic heart disease & Hypertension model. Using AADN-tables and originally developed computer program we can maintain within optimal ranges the intake with diet of 58 essential nutrients. Such a nutritional supervision on human being and the condition of caloric limitation will lead to an essential reduction of the rate of human aging. Panel of Experts - International Anti-Aging Expert Commission certified five values for each of 58 essential nutrients: A - minimum required, B - low optimum, I - ideal value, C - high optimum, D - unsafe level.

INTRODUCTION
Since the 1930s, caloric limitation of diet, y.e. dietary restriction(DR) has been the only intervention shown to slow aging in warm-blooded organisms. Early DR experiments of Clive M. McCay(1), who placed rats on a very low calorie diet and extended animal’s life span by 40 percent had been nowadays repeated in numerous experiments on mice and rats - for review see a comprehensive monograph of R. Weindruch and R.L. Walford(2). Mechanisms by which DR extend life span in rodents is now become more clear(3). DR decreases susceptibility to most diseases and significantly reduces aging rate in animals(4). There are ongoing trials of DR’s influence on aging in non-human primates(5,6). Preliminary observations furnished no proof that the quality of life of DR monkeys is impaired in any way. For human beings numerous low-calorie diets were introduced as an anti obese and therapeutic method. Low-calorie diets significantly improve various bio-markers of human aging(2,7,8,9). Even the American Dietetic Association, known for their conservatism, agreed with beneficial health role of low-calorie regimes for human beings(10).

Modern scientific data indicates that DR will with a high order of probability retard the rate of aging and extend life span in humans(2,7). Yet to achieve anti-aging goal low-calorie diets should be properly balanced in terms of all essential nutrients. Some of these nutrients - Vitamin E, Vitamin C, beta-Carotene, Selenium and others have intimate link with the aging process. Till now specialists on aging, dietitians and nutritionists have no unanimity of views upon: first - what nutrients are essential for anti-aging diet regimes, second - how much of these nutrients should be entered our organism daily with meals. In present work we tried to answer the questions what nutrients? and how much? As a result an anti-aging nutritional criteria - ANTI-AGING DIETARY NORMS(AADN) were developed. AADN have to serve as a standard guide for everybody, who want to control aging by nutritional methods.

MATERIAL AND METHODS

Definition
ANTI-AGING DIETARY NORMS are defined as the levels of intake of essential nutrients that the International Anti-Aging Expert Commission on the basis of scientific knowledge on biology of aging process, judges to be adequate and optimum for the maximum prevention of human aging process. The AADN are amounts intended to be consumed as part of a normal varied diet. AADN were developed for healthy persons (Standard version of AADN-tables) and for individuals with special health problems (Disease version of AADN-tables).

Philosophy
AADN-tables are intended to be a scientific and practical basis for gerontologists, nutritionists, dietetic practitioners and practical physicians, who deal with food planning as an anti-aging tool. Using AADN-tables one can prepare food rations in order to suppress human aging, diseases and obesity. AADN tables are in good correspondence with “US 10 Th. edition of the Recommended Dietary Allowances”, officially issued in 1989 by the Food and Nutrition Board of US National Academy of Sciences (1989 RDAs)(11). AADNs includes all 29 nutrients, that are presented in 1989 RDAs, but also contains other 29 essential nutrients. AADN are more complicated and diversified than 1989 RDAs. AADN are disease oriented tables. Certain types of health problems are correspondent to certain version of AADN-tables, that is in good correspondence with the recommendations of National Research Council(12) and of Americans Dietetic Association(10). AADN-tables are also in good agreement with the official recommendations of “The Surgeon General Report on Nutrition and Health”, that was published in 1989 by US Government(13).

Age/sex grouping
Although beneficiary effects of Anti-Aging food rations seems be achieved in any age group we consider that AADN-tables are developed for age groups starting from 20 years of age. We make no difference by sex in AADN-tables.

Design of AADN-tables
Generally every nutrient in AADNs tables is presented by five values: A- value (minimum required), B-value (low optimum), I-value (ideal value), C-value (high optimum), D-values (unsafe level). These values may differ for persons having different diseases or being on different caloric limitation regimes.

A-values (left column in AADN-tables) must be achieved while preparing daily meal plans. A values are to coincide with the corresponding male values of 1989 RDAs tables of age group 25-50 except Energy value. For those nutrients, that are not presented in 1989 RDAs A-, B-, I-, C- and D-values were developed by International Anti-Aging Expert Commission.

D-values (right column in AADN-tables) are corresponding to potentially undesirable level of nutrient intake or even to toxic values.

B- and C-values(two middle columns in AADN-tables) contains values of nutrients that indicate anti-aging optimum range for human beings. We consider that the rate of human aging for persons being on diet, when nutrients are within this optimum range (between B and C) is decreased to a minimum.

I-values (central column in AADN-tables) contains nutrient values, that are corresponding to the most desirable nutrient value. I-value’s position is between B- and C-values and its meaning is rather relative but serves as a basic figure for calculating of A-, B-, C- and D-values for some nutrients. AADN presents 5 values (A, B, I, C and D) for each of 58 essential nutrients that are combined into 6 subgroups: Main, Amino acids, Lipids, Carbohydrates, Vitamins and Elements. Methods of calculating of A-, B-, I-, C-, D-values for Standard model of AADN are presented below.

Main (include 8 nutrients)
Energy(E) (in kCal). Adequately adjusted daily energy intake has a crucial meaning for developing of anti-aging diet regimens. Although individual daily energy intake can be different depending on personal prescriptions, we used in present work 2000 kCal daily energy intake as a Standard value for all further calculations. A- and B-values are lower than the I-value by 6% and 3% correspondingly. C- and D-values are higher than I-value by 3% and 6% correspondingly. Thus for 2000 kCal regimes A-, B-, I-, C- and D-values should be 1880, 1940, 2000, 2060 and 2120 correspondingly (Table 1). AADN may have different versions depending on Energy content of a diet.

Proteins(Prot), Lipids(Lip), Carbohydrates(Carb) (as % of Energy). I-values for these nutrients are presented in Table 2 for different disease models. For Proteinsand Carbohydrates A- and B- values are lower than I-value by 30% and 20% correspondingly. C- and D-values are higher than I-value by 20% and 30% correspondingly. For Lipids A-, B-, C- and D-values are lower and higher than I-value by --60%, --50%, +20% and +50% correspondingly.

Indispensable Amino Acids (Total IAA) (as % of Energy). Total IAA’s daily intake is considered to be 33% of Total Protein Daily Intake(14). I-value for Total IAA is calculated by multiplying I-value for Proteins by 0,33. A-, B-, C- and D-values are lower and higher, than I-Total IAA value by --67%, --30% +50% and +100% correspondingly. A-value is correspondent to the recommendations of Food and Agriculture Organization FAO/WHO/UNU, 1985 (15).

Total fiber (in g) = Soluble fiber + cellulose + hemicellulose + lignin.

I-value for Total fiber is 25 g. A-, B-, C- and D-values are lower and higher than I-value by --30%, --20%, +20% and +40% correspondingly. Total fiber content should be decreased for persons with some gastrointestinal diseases, or for persons, who during long period of life were on low fiber food rations. (Gastroentheropathy version of AADN-tables).

Soluble fiber(FibS) (in g) = pectins + micilages + gums + algae&seaweeds + inulin.

I-value for Soluble fiber are calculated by multiplying of Total fiber’s I-value by 0,30. A-, B-, C- and D-values are lower and higher, than I-value by --30%, --20%, +20% and +40% correspondingly.

Water(H2O)(in g). I-value is 2000 g. A-, B-, C- and D-values are lower and higher than I-value by --25%, --15%, +15% and +25% correspondingly.

Amino acids (include 8 nutrients)
Amino acids include 8 nutrient parameters as % of Energy, that are corresponded to 10 Indispensable Amino Acids(IAA). We do not include Histidine in AADN-tables as well as indispensable statue of this amino-acid was proved only recently and requirements for this IAA is not yet established with certainty. However we took Histidine into account while calculating percentage ratio for certain IAA as we used the data from paper of P. Pellet, 1990 (16). If we consider 100% is total sum of IAA, than percentage ratio for certain amino-acid parameter(AA%) would be for Valine(Val) - 10,7%, for Isoleucine(Ile) - 10,7%, for Leucine(Leu) - 15,0%, for Lysine(Lys) - is 12,8%, for Methionine + Cystin(M+C) - 13,9%, for Threonine(Tre) - 7,5%, for Tryptophan(Trp) - 3,7%, for Phenylalanine + Tyrosine(P+T) - 15,0%, for Histidine - 10,7%. Ideal values for each of 8 IAA nutrient parameters are presented in AADN-tables as % of Energy (Table 1) and can be calculated by the following formula:

AA%E = Prot%E x 0.33 x AA% x 0.01 where

AA%E - I-value for certain AA parameter as % of Energy.

Prot%E - I-value for Protein as % of Energy.

AA% - percentage ratio for certain AA.

A-, B-, C- and D-values for certain AA parameter are lower and higher than I-value by --67%, --30%, +50%, +100%. For the convenience of calculations one can use the following formulas: A = I x 0.33, B = I x 0.7, C = I x 1.5, D = I x 2.0.

A-values are coincided with the recommendations of Food and Agricultural Organization (15).

Lipids (include 8 nutrient parameters)
Saturated fatty acids(SFA), Monounsaturated fatty acids(MFA)
and Polyunsaturated fatty acids(PFA)are presented in AADN-tables as % of Energy. I-values for these three nutrients are calculated by multiplying Lipids’ I-value by 0,20 for SFA, by 0,50 for MFA and by 0,30 for PFA. A-, B-, C- and D-values are lower and higher than I-values by

--50%, --25%, +50% and +100% correspondingly.

Linoleic acid(Lin)(as % of Energy). I-value is calculated by multiplying PFA’s I value by 0.75. A-, B-, C- and D-values are lower and higher, than I value by --50%, --25%, +50% and +100%.

Cholesterol(Chol)(in mg). I-value is 200 mg. A-, B-, C- and D-values are lower and higher, than I-value by --70%, --50%, +50% and +100% correspondingly.

PFA/SFA ratio(P/S). I-value is 1.50. A-, B-, C- and D-values are lower and higher than I value by --50%, --25%, +50% and +75%.

n-3/n-6 ratio(3/6).This is the ratio (n-3)PFA/(n-6)PFA. I-value is 0.33. A-, B-, C- and D-values are lower and higher, than I-value by

--75%, --50%, +100% and +200% correspondingly.

Alcohol(Alc)(as % of Energy). The consumption of alcohol is regarded to be safe, if not in access 1 or 2 drinks per day. That is why A-, B-, C- and D-values in grams are 0, 0, 30, 60. AADN-tables presents corresponding values as % of Energy. We consider, that energy content of 1 g of Alcohol is 7 kCal.

Carbohydrates (include 5 nutrient parameters),Vitamins (include 14 nutrients), Elements (include 15 nutrients).

A-, B-, C-, D-, I-values for these nutrients for Standard model are presented in Table 1.

RESULTS & DISCUSSION

Modern program
42 Versions of Anti-Aging Dietary Norms (food allowances) were developed by now in Anti-Aging Center, Budapest in a form of AADN-tables: 7 versions were developed for Standard model (900, 1200, 1500, 1700, 2000, 2200 and 2500 kCal), 9 versions - for Diabetic model (900, 1300, 1500, 1600, 1800, 2100, 2200, 2300 and 2400 kCal), 17 versions - for Gastroentheropaty model (900, 900LF, 1100, 1100LF, 1300, 1500, 1500LF, 1700, 1700LF, 1900, 1900LF, 2100, 2100LF, 2300, 2300LF, 2500 and 2500LF kCal. LF - means low-fiber content), 9 versions - for Ischemic Heart Disease & Hypertension model (1000, 1100, 1200, 1400, 1600, 1800, 1900, 2100 and 2300 kCal). All tables’ values are in good correspondence with modern views of diet - health relationships (17). Table 1 presents only 1(one) of 42(forty two) AADN-tables - 2000 kCal Standard model. Other 41(forty one) versions of AADNs are stored in Anti-Aging Center, Budapest.

A-, B-, I-, C- and D-values of AADN-tables are based on thorough investigation of modern scientific literature on biology of aging and human nutrition. We intentionally digress from discussing every of 58 particular nutrient figures included in Table 1 since we consider that present work will encourage professional discussion among nutritional experts, dietitians, biologists and gerontologists, who may revise these values. Every interested scientist is welcome to join our International Anti-Aging Expert Commission to revise and modify A-, B-, C- and D-values, presented in Table 1. as well as of values that are included in other 41 AADN-tables. Discussion on data presented in this paper have to yield a joint comprehensive view on the problem of designing universal anti-aging nutritional criteria - ANTI-AGING DIETARY NORMS and in final will provide public with a new and improved version of AADN-tables. AADN-tables have to be a theoretical basis for the developing of various anti-aging diet rations.

Original computer program was developed in Anti-Aging Center, Budapest to help design Personal Diet Plans. Using certain version of AADN and with the help of food composition list a trained computer’s user can readily design individually adjusted Anti-Aging Diet Plan. Results of such a design for 2000 kCal Standard model version of AADN are presented on fig. 1. Nutrient’s composition of this Diet Plan is presented on fig 2. This Diet Plan (Fig. 1.) is only one of many possible computer’s work outs. Using AADN-tables anyone can be challenged to design their own individual Diet Plans according to their own personal taste. Recipes, included in Diet Plan are easy and fast cooking. Usually when daily energy intake is lower than 2300 - 2500 kCal Diet Plan must be enriched by vitamin and mineral supplements. In the Anti-Aging Diet Plan presented on Fig. 1 majority of 58 essential nutrients are being within the optimum range - between Low Optimum value (B) and High Optimum value(C). One can found from Fig. 2 that 49 of 58 nutrient parameters (85% of nutrients that were monitored) are lying within the Anti-Aging optimum range. We consider that in order to achieve adequate anti-aging protective effect of diet it is quite sufficient that vitamins and elements (totally 29) have to be between “minimum required value” (A) and “high optimum value” (C). Other nutrients - main, amino-acids, lipids, carbohydrates (totally 29) - should be more strictly balanced within Anti-Aging optimum range (between values B and C). Generally when 3/4 of nutrients are within optimum anti-aging range such a diet is regarded to be a diet with high degree of “balancing quality”.

Diet Plan (Fig. 1) can be used by an average healthy person, who wants to improve its health status and decrease the rate of aging. Individuals, aggravated with certain chronic diseases or who predispose to certain type of pathology should use other than Standard model version of AADN-tables. Due to lack of space 41 versions of AADN are not presented in present paper.

After approvement and verification of AADN-tables by anti-aging specialists, dietitians, nutritionists, physicians, and other practitioners everybody can readily and with confidence use AADN-tables as a practical guidance for designing individually adjusted Anti-Aging Diet Plans.

Future program
Control on human aging can be achieved on two levels of supervision on entry to the body (entry monitoring) and inside the body (inside monitoring). In present paper we attempted to develop a method of control of essential nutrients enter our organism (entry monitoring). Next step one have to supervise essential biochemical entities inside the body (inside monitoring). Due to differences in genetics, individual physiology, physical activity, nutrient bioavailability, predisposition to certain diseases and due to environmental differences or style of living it is more desirable to check all essential nutrients inside human body (inside monitoring). We mean that blood composition must be analyzed with a view to maintain within optimum anti-aging ranges the concentration of the maximum possible number of nutrients - amino acids, carbohydrates, lipoproteins, cholesterol, vitamins, micro and macro-elements, as well as of the most important biologically active substances: hormones, proteins and other essential endogenic species. We must monitor the quality and quantity of human biochemical media. In case the parameters monitored happen to be beyond the special optimum limits, appropriate corrections will be made by way of purpose-oriented changes in the character of the diet, exercises or other factors. If necessary vitamins, minerals, geroprotectors, nootropes and other supplements may be introduced into the Anti-Aging Diet Plan. The precise supervision on human being (entry and inside monitoring) as well as the condition of caloric limitation undoubtedly will lead to an essential reduction of the rate of human aging and essential prolongation of human life span.

ACKNOWLEDGMENTS
This work was supported by Anti-Aging Center Inc. The authors wish to extend their appreciation to Dr. Roy L. Walford, Professor of the UCLA School of Medicine for his extremely valuable monographs on the dietary restriction and aging, that encouraged us to perform this work. We are grateful to my colleagues and experts for their valuable contribution and criticism, while preparing present 1-St. Edition of Anti-Aging Dietary Norms.

REFERENCES
1. McCay C.M., Sperling G., Barnes L.L.: Growth, ageing, chronic diseases and life span in rats. Arch. Biochem. 2: 469-479, 1943.
2. Weindruch R., Walford R.L.: The Retardation of Aging and Diseases by Dietary Restriction. Charles C Thomas, Springfield, IL, 1988, pp. 1-436.
3. Masoro E.J., McCarter R.J.M., Katz M.S., McMahan C.A.: Dietary restriction alters characteristics of glucose fuel use. J. Gerontol. Biol. Sci. 47: B202-B208, 1992.
4. Holehan A.M., Merry B.J.: The experimental manipulation of ageing by diet. Biol. Rev. 61: 329 - 368, 1986.
5. Ingram D.K., Cutler R.G., Weindruch R., Renquist D.M., Knapka J.J., April M., Belcher C.T., Clark M.A., Hatcherson C.D., Marriott B.M., Roth G.S.: Dietary restriction and aging: The initiation of primate study. J. Gerontol. Biol. Sci. 45: B148-B163, 1990.
6. Kemnitz J.W., Weindruch R., Roecker E.B., Crawford K., Kaufman P.L., Ershler W.B.: Dietary restriction of adult male rhesus monkeys: design, methodology, and preliminary findings from the first year of study. J. Gerontol. Biol. Sci. 48: B17-B26, 1993.
7. Anderson J.W., Brinkman V.L., Hamilton C.C.: Weight loss and 2-y follow-up for 80 morbidly obese patients treated with intensive very-low-calorie diet and an education program. Am. J. Clin. Nutr. 56: 244S-246S, 1992.
8. Walford R.L., Harris S.B., Gunion M.W.: The calorically restricted low-fat nutrient-dense diet in Biosphere 2 significantly lowers blood glucose, total leukocyte count, cholesterol, and blood pressure in humans. Proc. Natl. Acad. Sci. USA. 89: 11533-11537, 1992.
9. Hainer V., Stich V., Kunesová M., Parizková J., Zák A., Wernischová V., Hrabák P.: Effect of 4-wk treatment of obesity by very-low-calorie diet on anthropometric, metabolic, and hormonal indexes. Am. J. Clin. Nutr. 56: 281S-282S, 1992.
10. American Dietetic Association.: Position of the American dietetic association: very-low-calorie weight loss diets. J. Am. Diet. Assoc. 90: 722-726, 1990.
11. Food and Nutrition Board, Committee on Dietary Allowances, National Research Council: Recommended Dietary Allowances, 10th rev. ed.: Natl. Acad. Sci., Washington, DC, 1989.
12. National Research Council: Diet and health: implication for reducing chronic disease risk, National Academy Press, Washington, DC, 1989.
13. US Government. Department of Health and Human Services.: The Surgeon General’s Report on Nutrition and Health. Washington, DC 1988.
14. Young V.R., Bier D.M.: Amino acid requirements in the adult human. How well do we know them? J. Nutr. 117: 1484-1487, 1987.
15. Food and Agricultural Organization. FAO/WHO/UNO Committee.: Energy and protein requirements. World Health Organization, Geneva, 1985. (WHO technical report series #724).
16. Pellet P.L.: Protein requirements in humans. Am. J. Clin. Nutr. 51: 723-737, 1990.
17. Willett W.C.: Diet and health: what should we eat? Science 264: 532-537, 1994.

Table 1. Anti-Aging Dietary Norm-table, 2000 kCal Standard model.

Nutrients
A
B
I
C
D
minimum
required
low
optimum
ideal
value

high
optimum

unsafe
level
MAIN
         
1.
Energy (E)
kCal
1880.0
1940.0
2000.0
2060.0
2120.0
2.
Proteins (Prot)
%of E
10.5
12.0
15.0
18.0
19.5
3.
Lipids (Lip)
%of E
8.0
10.0
20.0
24.0
30.0
4.
Carbohydrates (Carb)
%of E
45.5
52.0
65.0
78.0
84.5
5.
Sum of Indispensable Amino Acids(IAA)
%of E
1.63
3.47
4.95
7.43
9.90
6.
Fiber-total (Fib)
g
17.5
20.0
25.0
30.0
35.0
7.
Fiber-sulible (FibS)
g
5.25
6.0
7.5
9.0
10.5
8.
Water (H20)
g
1500.0
1700.0
2000.0
2300.0
2500.0
AMINO ACIDS
9.
Valine (Val)
%of E
0.18
0.37
0.53
0.79
1.06
10.
Isoleucine (Ile)
%of E
0.18
0.37
0.53
0.79
1.06
11.
Leucine (Leu)
%of E
0.25
0.52
0.74
1.11
1.49
12.
Lysine (Lys)
%of E
0.21
0.44
0.64
0.95
1.27
13.
Methionine & Cystine (M+C)
%of E
0.23
0.48
0.69
1.03
1.38
14.
Threonine (Tre)
%of E
0.12
0.26
0.37
0.56
0.74
15.
Tryptophan (Trp)
%of E
0.06
0.13
0.18
0.28
0.37
16.
Phenylalanine & Tyrosine (P+T)
%of E
0.25
0.52
0.74
1.12
1.49
LIPIDS
17.
Saturated fatty acids (SFA)
%of E
2.0
3.0
4.0
6.0
8.0
18.
Monounsaturated fatty acid (MFA)
%of E
5.0
7.5
10.0
15.0
20.0
19.
Polyunsaturated fatty acids (PFA)
%of E
3.0
4.5
6.0
9.0
12.0
20.
Linoleic acid (Lin)
%of E
2.25
3.385
4.50
6.75
9.00
21.
Cholesterol (Chol)
mg
60.
100.0
200.0
300.0
400.0
22.
PFA/SFA ratio (P/S)
0.75
1.13
1.50
2.25
2.63
23.
n-3/n-6 ratio (3/6)
0.08
0.17
0.33
0.66
0.99
24.
Alcohol (Alc)
%of E
0.0
1.0
5.75
10.5
21.0
CARBOHYDRATES
25.
Glucose(Glu)
%of E
0.0
1.0
4.5
8.0
10.0
26.
Sucrose(Suc)
%of E
0.0
1.0
7.0
13.0
16.0
27.
Lactose(Lac)
%of E
0.0
1.0
3.0
5.0
8.0
28.
Fructose(Fru)
%of E
0.0
1.0
5.5
10.0
15.0
29
Starch & Dextrin (S+D)
%of E
0.0
1.0
28.0
57.0
68.0
VITAMINS
30.
beta-Carotene (b-C)
mcg
4200.0
4800.0
9900.0
15000.0
45000.0
31.
Vitamin A (A)
mcg, RE
1000.0
1000.0
1750.0
2500.0
7500.0
32.
Vitamin D (D)
mcg
5.0
10.0
15.0
20.0
50.0
33.
Vitamin K (K)
mcg
45.0
70.0
85.0
100.0
300.0
34.
Vitamin E (E)
mcg, TE
10.0
200.0
300.0
400.0
800.0
35.
Vitamin C (C)
mg
60.0
250.0
375.0
500.0
1000.0
36.
Vitamin B-1 (B-1)
mg
1.5
3.0
11.5
20.0
200.0
37.
Vitamin B-2 (B-2)
mg
1.7
5.0
12.5
20.0
1000.0
38.
Vitamin PP (PP)
mg
19.0
40.0
70.0
100.0
300.0
39.
Pantothenic acid (Pan)
mg
4.0
10.0
105.0
200.0
1000.0
40.
Vitamin B-6 (B-6)
mg
2.0
20.0
30.0
40.0
100.0
41.
Folic acid (Fol)
mcg
200.0
400.0
700.0
1000.0
4000.0
42.
Biotin (Bio)
mcg
30.0
200.0
400.0
600.0
5000.0
43.
Vitamin B-12 (B-12)
mcg
3.0
10.0
30.0
50.0
1000.0
ELEMENTS
44.
Potassium (K)
mg
2000.0
3000.0
4500.0
6000.0
8000.0
45.
Sodium (Na)
mg
500.0
500.0
1250.0
2000.0
3000.0
46.
Chlorine (Cl)
mg
750.0
750.0
1500.0
2250.0
4500.0
47.
Phosphorous (P)
mg
800.0
800.0
1000.0
1200.0
2000.0
48.
Calcium (Ca)
mg
800.0
1400.0
1700.0
2000.0
3000.0
49.
Magnesium (Mg)
mg
350.0
600.0
900.0
1200.0
2000.0
50.
Zinc (Zn)
mg
15.0
25.0
32.5
40.0
70.0
51.
Iron (Fe)
mg
15.0
18.0
21.5
25.0
50.0
52.
Manganese (Mn)
mg
2.0
5.0
7.5
10.0
30.0
53.
Copper (Cu)
mg
1.5
3.0
4.5
6.0
15.0
54.
Fluoride (F)
mcg
1500.0
1500.0
2750.0
4000.0
4000.0
55.
Iodine (I)
mcg
150.0
250.0
1125.0
2000.0
5000.0
56.
Molybdenum (Mo)
mcg
75.0
250.0
625.0
1000.0
2500.0
57.
Selenium (Se)
mcg
70.0
200.0
250.0
300.0
600.0
58.
Chromium (Cr)
mcg
50.0
200.0
300.0
400.0
600.0

Table 2. AADN’s I values for Proteins, Lipids, Carbohydrates for different disease models: Standard, Gastroentheropathy, Ischemic heart disease & Hypertension, Diabetic.



Figure 1. Anti-Aging Diet Plan for One Day. Sample computer’s work out for 2000 kCal Standard model.


Figure 2. “Balancing quality” of One Day Anti-Aging Diet Plan. 2000 kCal Standard Model.

A B C D
Nutrient
Current
Under minimum
required range
Below optimum
renage
Anti-Aging
optimum range
Above optimum range
Unsafe range
Energy (E)
kCal
1947.2
  ********  
Prot
%of E
17.4
  ********  
Lip
%of E
21.8
  *********  
Carb
%of E
61.5
  *********  
Total IAA
%of E
5.5
  *********  
Total fib
g
26.7
  *********  
Solub. fib
g
7.4
  *********  
Water
g
1704.5
  *********  
     
Val
%of E
0.72
  *********  
Ile
%of E
0.59
  *********  
Leu
%of E
1.06
  *********  
Lys
%of E
0.83
  *********  
M+C
%of E
0.51
  *********  
Tre
%of E
0.57
    *
Trp
%of E
0.16
  *********  
P+T
%of E
1.11
  *********  
     
SFA
%of E
3.6
  *********  
MFA
%of E
8.1
  *********  
PFA
%of E
7.3
  *********  
Lin
%of E
6.4
  *********  
Chol
mg
141.7
  *********  
PFA/SFA
2.0
  *********  
n-3/n-6
0.1
*******    
Alcohol
%of E
1.2
  *********  
     
Glu
%of E
7.7
  *********  
Suc
%of E
5.1
  *********  
Lac
%of E
2.2
  *********  
Fru
%of E
8.3
  *********  
S+D
%of E
31.6
  *********  
     
b-C
mcg
7725
  *********  
A
mcg, RE
1272
  *********  
D
mcg
18
  *********  
K
mcg
96
  *********  
E
mcg, TE
249
  *********  
C
mg
359
  *********  
B-1
mg
4
  *********  
B-2
mg
6
  *********  
PP
mg
45
  *********  
Pan
mg
19
  *********  
B-6
mg
6
*******    
Fol
mcg
840
  **********  
Bio
mcg
75
*******    
B-12
mcg
12
  **********  
     
K
mg
4648
  **********  
Na
mg
1592
  **********  
Cl
mg
1575
  **********  
P
mg
1640
    *******
Ca
mg
1343
********    
Mg
mg
620
  **********  
Zn
mg
31
  **********  
Fe
mg
43
    ********
Mn
mg
8
  **********  
Cu
mg
5
  ***********  
F
mcg
2814
  **********  
I
mcg
446
  *********  
Mo
mcg
113
******    
Se
mcg
219
  ********  
Cr
mcg
149
*******/    
               
   
   
   
 
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FASTING / LOW CALORIE PROGRAMS
on the Adriatic Coast
The Anti-Aging Fasting Program consists of a 7-28 days program (including 3 - 14 fasting days). 7-28-day low-calorie diet program is also available .
More information
    The anti-aging story (summary)
Introduction. Statistical review. Your personal aging curve
  Aging and Anti-aging. Why do we age?
    2.1  Aging forces (forces that cause aging
     
Internal (free radicals, glycosylation, chelation etc.) 
External (Unhealthy diet, lifestyle, wrong habits, environmental pollution, stress, poverty-change "poverty zones", or take it easy. etc.) 
    2.2 Anti-aging forces
     
Internal (apoptosis, boosting your immune system, DNA repair, longevity genes) 
External (wellness, changing your environment; achieving comfortable social atmosphere in your life, regular intake of anti-aging drugs, use of replacement organs, high-tech medicine, exercise)
    2.3 Aging versus anti-aging: how to tip the balance in your favour!
 
    3.1 Caloric restriction and fasting extend lifespan and decrease all-cause mortality (Evidence)
      Human studies
Monkey studies
Mouse and rat studies
Other animal studies
    3.2 Fasting and caloric restriction prevent and cure diseases (Evidence)
        Obesity
Diabetes
Hypertension and Stroke
Skin disorders
Mental disorders
Neurogical disorders
Asthmatic bronchitis, Bronchial asthma
Bones (osteoporosis) and fasting
Arteriosclerosis and Heart Disease
Cancer and caloric restriction
Cancer and fasting - a matter of controversy
Eye diseases
Chronic fatigue syndrome
Sleeping disorders
Allergies
Rheumatoid arthritis
Gastrointestinal diseases
Infertility
Presbyacusis
    3.3 Fasting and caloric restriction produce various
      biological effects. Effects on:
        Energy metabolism
Lipids metabolism
Protein metabolism and protein quality
Neuroendocrine and hormonal system
Immune system
Physiological functions
Reproductive function
Radio-sensitivity
Apoptosis
Cognitive and behavioral functions
Biomarkers of aging
    3.4 Mechanisms: how does calorie restriction retard aging and boost health?
        Diminishing of aging forces
  Lowering of the rate of gene damage
  Reduction of free-radical production
  Reduction of metabolic rate (i.e. rate of aging)
  Lowering of body temperature
  Lowering of protein glycation
Increase of anti-aging forces
  Enhancement of gene reparation
  Enhancement of free radical neutralisation
  Enhancement of protein turnover (protein regeneration)
  Enhancement of immune response
  Activation of mono-oxygenase systems
  Enhance elimination of damaged cells
  Optimisation of neuroendocrine functions
    3.5 Practical implementation: your anti-aging dieting
        Fasting period.
Re-feeding period.
Safety of fasting and low-calorie dieting. Precautions.
      3.6 What can help you make the transition to the low-calorie life style?
        Social, psychological and religious support - crucial factors for a successful transition.
Drugs to ease the transition to caloric restriction and to overcome food cravings (use of adaptogenic herbs)
Food composition
Finding the right physician
    3.7Fasting centers and fasting programs.
  Food to eat. Dishes and menus.
    What to eat on non-fasting days. Dishes and menus. Healthy nutrition. Relation between foodstuffs and diseases. Functional foods. Glycemic index. Diet plan: practical summary. "Dr. Atkins", "Hollywood" and other fad diets versus medical science
     

Vegetables
Fruits
Bread, cereals, pasta, fiber
Glycemic index
Fish
Meat and poultry
Sugar and sweet
Legumes
Fats and oils
Dairy and eggs
Mushrooms
Nuts and seeds
Alcohol
Coffee
Water
Food composition

  Anti-aging drugs and supplements
    5.1 Drugs that are highly recommended
      (for inclusion in your supplementation anti-aging program)
        Vitamin E
Vitamin C
Co-enzyme Q10
Lipoic acid
Folic acid
Selenium
Flavonoids, carotenes
DHEA
Vitamin B
Carnitin
SAM
Vinpocetine (Cavinton)
Deprenyl (Eldepryl)
    5.2 Drugs with controversial or unproven anti-aging effect, or awaiting other evaluation (side-effects)
        Phyto-medicines, Herbs
HGH
Gerovital
Melatonin
      5.3 Drugs for treatment and prevention of specific diseases of aging. High-tech modern pharmacology.
        Alzheimer's disease and Dementia
Arthritis
Cancer
Depression
Diabetes
Hyperlipidemia
Hypertension
Immune decline
Infections, bacterial
Infections, fungal
Memory loss
Menopause
Muscle weakness
Osteoporosis
Parkinson's disease
Prostate hyperplasia
Sexual disorders
Stroke risk
Weight gaining
    5.4 The place of anti-aging drugs in the whole
      program - a realistic evaluation
 
    6.1 Early diagnosis of disease - key factor to successful treatment.
      Alzheimer's disease and Dementia
Arthritis
Cancer
Depression
Diabetes
Cataracts and Glaucoma
Genetic disorders
Heart attacks
Hyperlipidemia
Hypertension
Immune decline
Infectious diseases
Memory loss
Muscle weakness
Osteoporosis
Parkinson's disease
Prostate hyperplasia
Stroke risk
Weight gaining
    6.2 Biomarkers of aging and specific diseases
    6.3 Stem cell therapy and therapeutic cloning
    6.4 Gene manipulation
    6.5 Prosthetic body-parts, artificial organs
        Blood
Bones, limbs, joints etc.
Brain
Heart & heart devices
Kidney
Liver
Lung
Pancreas
Spleen
    6.6 Obesity reduction by ultrasonic treatment
  Physical activity and aging. Experimental and clinical data.
        Aerobic exercises
Stretching
Weight-lifting - body-building
Professional sport: negative aspects
 
  Conclusion: the whole anti-aging program
    9.1 Modifying your personal aging curve
      Average life span increment. Expert evaluation.
     
Periodic fasting and caloric restriction can add 40 - 50 years to your lifespan
Regular intake of anti-aging drugs can add 20-30 years to your lifespan
Good nutrition (well balanced, healthy food, individually tailord diet) can add 15-25 years to your lifespan
High-tech bio-medicine service can add 15-25 years to your lifespan
Quality of life (prosperity, relaxation, regular vocations) can add 15-25 years to your lifespan
Regular exercise and moderate physical activity can add 10-20 years to your lifespan
These approaches taken together can add 60-80 years to your lifespan, if you start young (say at age 20). But even if you only start later (say at 45-50), you can still gain 30-40 years


Click image
to view
    9.2 The whole anti-aging life style - brief summary 
    References eXTReMe Tracker
        The whole anti-aging program: overview
         
       

       
     
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